Analysis Note

ControlCRTC1-hIgGFc transfected HEK293 cell lysate

Application

Research CategoryApoptosis & Cancer

Detect CREB-regulated transcription coactivator 2 using this mouse monoclonal antibody, Anti-CRTC1 Antibody, clone 1B5 validated for use in western blotting, ICC & Flow Cytometry.

Research Sub CategoryApoptosis - Additional

Immunocytochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected CRTC1 in U251 and NTERA2 cells.

Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected CRTC1 in K562 cells.

Optimal working dilutions must be determined by end user.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

CREB-regulated transcription coactivator 1 (CRTC1) or in older literature, Mucoepidermoid carcinoma translocated protein 1 or Transducer of regulated cAMP response element-binding element 1 or Transducer of regulated CREB activity 1 (TORC1), is encoded by the human gene named CRTC1/KIAA0616/MECT1, TORC1, or WAMTP1, and CRTC1 is a transcriptional coactivator for CREB1 protein which activates transcription through binding cAMP response element (CRE) sites on DNA. CRTC1 appears to interact with many signaling and metabolic pathways through its regulation of CREB and thus, CRTC1 is involved in diverse physiological processes including energy homeostasis, cancer and endoplasmic reticulum stress. CRTC1 is a powerful activator of PGC1alpha expression and critical for mitochondrial biogenesis in muscle cells. CRTC1 function also varies by cell type. For example, in muscle cells CRTC1 is critical for mitochondrial development, in hippocampal neurons CRTC1 is important for the maintenance of L-LTP (late phase long-term potentiation), and in cortical neurons CRTC1 is important for dendritic spine growth and early neuronal development. CRTC1 is nuclear localized when dephosphorylated and active. CRTC1 is widely expressed in tissues but highest in developing fetal and adult brain, specifically prefrontal cortex and cerebellum. Interestingly recent research on CRTC1 in worms suggests that inhibiting CRTC1 either through phosphorylation or translation extends lifespan significantly, and thus CRTC1 may be important in combating aging and its effects as well. EMD-Millipore Anti-CRTC1 mouse monoclonal antibody has been tested in western blot against recombinant CRTC1 protein and in fluorescent immunocytochemistry on U251 and NTERA2 cell in culture. In addition the antibody has been successfully tested in flow cytometry on K562 cells in culture.

Immunogen

Purified recombinant fragment of human CRTC1 expressed in E. Coli.

Physical form

Mouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.

Antigen Affinity Purified

Quality

Evaluated by Western Blotting in CRTC1-hIgGFc transfected HEK293 cell lysate.

Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected CRTC1 in CRTC1-hIgGFc transfected HEK293 cell lysate.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

~67 kDa observed. Uncharacterized bands may appear in some lysate(s). Uniprot describes 3 isoforms between ~63 kDa and ~69 kDa